photon microscope Search Results


twophoton microscope  (Thorlabs)
86
Thorlabs twophoton microscope
Twophoton Microscope, supplied by Thorlabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/photon+microscope/microscope+photon+two/pm41391146-317-6-17
Average 86 stars, based on 1 article reviews
twophoton microscope - by Bioz Stars, 2026-08
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two-photon benchtop microscope  (Femtonics inc)
90
Femtonics inc two-photon benchtop microscope
A) Schematic of experimental design and resulting histology 24 days after the rabies injection. Top left: a population of birthdate-matched (E14) cells in the hippocampus was targeted to express TVA and G by injecting an undiluted virus carrying Cre in utero and a large volume of the rabies helper virus carrying TVA and G in the adult hippocampus. A rabies virus carrying GCaMP6f was used to label cells providing monosynaptic input to this cell population. These inputs were then visualised via a prism implanted between the MEC (brain region marked in grey) and the cerebellum. Bottom left: confocal maximum intensity projection of the dorsal hippocampus showing cells expressing helper proteins and/or GCaMP6f. Image insets are single z-plane confocal images showing colocalization of antibody staining for a tag introduced by the helper virus and GCaMP6f introduced by the rabies virus in one example cell (i.e. a starter cell) and only GCaMP6f expression in a neighbouring cell (i.e. an input cell) highlighted with an arrow and a yellow dotted circle. Right: confocal maximum intensity projection showing input cells expressing GCaMP6f in the MEC. The white dotted line illustrates the impression made by the prism on the MEC. DG: dentate gyrus, Sub: subiculum. B) In vivo imaging example. Top left: imaging is performed with head-fixed mice under a two-photon <t>benchtop</t> <t>microscope.</t> Right: maximum intensity projection (across a 2-minute recording) of five cells in the MEC that provide monosynaptic input to the ipsilateral hippocampus 19 days after the rabies injection. Bottom left: ΔF/F traces of two cells with different activity onsets highlighted with corresponding colours in the image to the right.
Two Photon Benchtop Microscope, supplied by Femtonics inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/photon+microscope/two+photon+benchtop+microscope/bio_rxiv__2021__10__18__464781-265-6-9
Average 90 stars, based on 1 article reviews
two-photon benchtop microscope - by Bioz Stars, 2026-08
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photometer d-104  (Photon Technology International Inc)
90
Photon Technology International Inc photometer d-104
A) Schematic of experimental design and resulting histology 24 days after the rabies injection. Top left: a population of birthdate-matched (E14) cells in the hippocampus was targeted to express TVA and G by injecting an undiluted virus carrying Cre in utero and a large volume of the rabies helper virus carrying TVA and G in the adult hippocampus. A rabies virus carrying GCaMP6f was used to label cells providing monosynaptic input to this cell population. These inputs were then visualised via a prism implanted between the MEC (brain region marked in grey) and the cerebellum. Bottom left: confocal maximum intensity projection of the dorsal hippocampus showing cells expressing helper proteins and/or GCaMP6f. Image insets are single z-plane confocal images showing colocalization of antibody staining for a tag introduced by the helper virus and GCaMP6f introduced by the rabies virus in one example cell (i.e. a starter cell) and only GCaMP6f expression in a neighbouring cell (i.e. an input cell) highlighted with an arrow and a yellow dotted circle. Right: confocal maximum intensity projection showing input cells expressing GCaMP6f in the MEC. The white dotted line illustrates the impression made by the prism on the MEC. DG: dentate gyrus, Sub: subiculum. B) In vivo imaging example. Top left: imaging is performed with head-fixed mice under a two-photon <t>benchtop</t> <t>microscope.</t> Right: maximum intensity projection (across a 2-minute recording) of five cells in the MEC that provide monosynaptic input to the ipsilateral hippocampus 19 days after the rabies injection. Bottom left: ΔF/F traces of two cells with different activity onsets highlighted with corresponding colours in the image to the right.
Photometer D 104, supplied by Photon Technology International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/photon+microscope/microscope+photometer/pmc03026912-142-14-17
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photometer d-104 - by Bioz Stars, 2026-08
90/100 stars
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scanning electron microscope images of a completed hcg vcsel  (Optics and Photonics)
90
Optics and Photonics scanning electron microscope images of a completed hcg vcsel
A) Schematic of experimental design and resulting histology 24 days after the rabies injection. Top left: a population of birthdate-matched (E14) cells in the hippocampus was targeted to express TVA and G by injecting an undiluted virus carrying Cre in utero and a large volume of the rabies helper virus carrying TVA and G in the adult hippocampus. A rabies virus carrying GCaMP6f was used to label cells providing monosynaptic input to this cell population. These inputs were then visualised via a prism implanted between the MEC (brain region marked in grey) and the cerebellum. Bottom left: confocal maximum intensity projection of the dorsal hippocampus showing cells expressing helper proteins and/or GCaMP6f. Image insets are single z-plane confocal images showing colocalization of antibody staining for a tag introduced by the helper virus and GCaMP6f introduced by the rabies virus in one example cell (i.e. a starter cell) and only GCaMP6f expression in a neighbouring cell (i.e. an input cell) highlighted with an arrow and a yellow dotted circle. Right: confocal maximum intensity projection showing input cells expressing GCaMP6f in the MEC. The white dotted line illustrates the impression made by the prism on the MEC. DG: dentate gyrus, Sub: subiculum. B) In vivo imaging example. Top left: imaging is performed with head-fixed mice under a two-photon <t>benchtop</t> <t>microscope.</t> Right: maximum intensity projection (across a 2-minute recording) of five cells in the MEC that provide monosynaptic input to the ipsilateral hippocampus 19 days after the rabies injection. Bottom left: ΔF/F traces of two cells with different activity onsets highlighted with corresponding colours in the image to the right.
Scanning Electron Microscope Images Of A Completed Hcg Vcsel, supplied by Optics and Photonics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/photon+microscope/scanning+electron+microscope+images+of+a+completed+hcg+vcsel/10__1364_slash_aop__4__000379-500-24-2
Average 90 stars, based on 1 article reviews
scanning electron microscope images of a completed hcg vcsel - by Bioz Stars, 2026-08
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raman microscope nd:yag laser  (TOPTICA Photonics)
90
TOPTICA Photonics raman microscope nd:yag laser
A) Schematic of experimental design and resulting histology 24 days after the rabies injection. Top left: a population of birthdate-matched (E14) cells in the hippocampus was targeted to express TVA and G by injecting an undiluted virus carrying Cre in utero and a large volume of the rabies helper virus carrying TVA and G in the adult hippocampus. A rabies virus carrying GCaMP6f was used to label cells providing monosynaptic input to this cell population. These inputs were then visualised via a prism implanted between the MEC (brain region marked in grey) and the cerebellum. Bottom left: confocal maximum intensity projection of the dorsal hippocampus showing cells expressing helper proteins and/or GCaMP6f. Image insets are single z-plane confocal images showing colocalization of antibody staining for a tag introduced by the helper virus and GCaMP6f introduced by the rabies virus in one example cell (i.e. a starter cell) and only GCaMP6f expression in a neighbouring cell (i.e. an input cell) highlighted with an arrow and a yellow dotted circle. Right: confocal maximum intensity projection showing input cells expressing GCaMP6f in the MEC. The white dotted line illustrates the impression made by the prism on the MEC. DG: dentate gyrus, Sub: subiculum. B) In vivo imaging example. Top left: imaging is performed with head-fixed mice under a two-photon <t>benchtop</t> <t>microscope.</t> Right: maximum intensity projection (across a 2-minute recording) of five cells in the MEC that provide monosynaptic input to the ipsilateral hippocampus 19 days after the rabies injection. Bottom left: ΔF/F traces of two cells with different activity onsets highlighted with corresponding colours in the image to the right.
Raman Microscope Nd:Yag Laser, supplied by TOPTICA Photonics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/photon+microscope/raman+microscope+nd+yag+laser/pm24712726-29-25-32
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raman microscope nd:yag laser - by Bioz Stars, 2026-08
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two-photon microscope dermainspect  (JenLab GmbH)
90
JenLab GmbH two-photon microscope dermainspect
A) Schematic of experimental design and resulting histology 24 days after the rabies injection. Top left: a population of birthdate-matched (E14) cells in the hippocampus was targeted to express TVA and G by injecting an undiluted virus carrying Cre in utero and a large volume of the rabies helper virus carrying TVA and G in the adult hippocampus. A rabies virus carrying GCaMP6f was used to label cells providing monosynaptic input to this cell population. These inputs were then visualised via a prism implanted between the MEC (brain region marked in grey) and the cerebellum. Bottom left: confocal maximum intensity projection of the dorsal hippocampus showing cells expressing helper proteins and/or GCaMP6f. Image insets are single z-plane confocal images showing colocalization of antibody staining for a tag introduced by the helper virus and GCaMP6f introduced by the rabies virus in one example cell (i.e. a starter cell) and only GCaMP6f expression in a neighbouring cell (i.e. an input cell) highlighted with an arrow and a yellow dotted circle. Right: confocal maximum intensity projection showing input cells expressing GCaMP6f in the MEC. The white dotted line illustrates the impression made by the prism on the MEC. DG: dentate gyrus, Sub: subiculum. B) In vivo imaging example. Top left: imaging is performed with head-fixed mice under a two-photon <t>benchtop</t> <t>microscope.</t> Right: maximum intensity projection (across a 2-minute recording) of five cells in the MEC that provide monosynaptic input to the ipsilateral hippocampus 19 days after the rabies injection. Bottom left: ΔF/F traces of two cells with different activity onsets highlighted with corresponding colours in the image to the right.
Two Photon Microscope Dermainspect, supplied by JenLab GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/photon+microscope/two+photon+microscope+dermainspect/pmc03136453-153-1-4
Average 90 stars, based on 1 article reviews
two-photon microscope dermainspect - by Bioz Stars, 2026-08
90/100 stars
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2d two-photon laser scanning microscope  (Femtonics inc)
90
Femtonics inc 2d two-photon laser scanning microscope
A) Schematic of experimental design and resulting histology 24 days after the rabies injection. Top left: a population of birthdate-matched (E14) cells in the hippocampus was targeted to express TVA and G by injecting an undiluted virus carrying Cre in utero and a large volume of the rabies helper virus carrying TVA and G in the adult hippocampus. A rabies virus carrying GCaMP6f was used to label cells providing monosynaptic input to this cell population. These inputs were then visualised via a prism implanted between the MEC (brain region marked in grey) and the cerebellum. Bottom left: confocal maximum intensity projection of the dorsal hippocampus showing cells expressing helper proteins and/or GCaMP6f. Image insets are single z-plane confocal images showing colocalization of antibody staining for a tag introduced by the helper virus and GCaMP6f introduced by the rabies virus in one example cell (i.e. a starter cell) and only GCaMP6f expression in a neighbouring cell (i.e. an input cell) highlighted with an arrow and a yellow dotted circle. Right: confocal maximum intensity projection showing input cells expressing GCaMP6f in the MEC. The white dotted line illustrates the impression made by the prism on the MEC. DG: dentate gyrus, Sub: subiculum. B) In vivo imaging example. Top left: imaging is performed with head-fixed mice under a two-photon <t>benchtop</t> <t>microscope.</t> Right: maximum intensity projection (across a 2-minute recording) of five cells in the MEC that provide monosynaptic input to the ipsilateral hippocampus 19 days after the rabies injection. Bottom left: ΔF/F traces of two cells with different activity onsets highlighted with corresponding colours in the image to the right.
2d Two Photon Laser Scanning Microscope, supplied by Femtonics inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/photon+microscope/2d+two+photon+laser+scanning+microscope/pmc08075564-45-10-9
Average 90 stars, based on 1 article reviews
2d two-photon laser scanning microscope - by Bioz Stars, 2026-08
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custom-built laserscanning multiphoton microscope  (Laserscanning Europe GmbH)
90
Laserscanning Europe GmbH custom-built laserscanning multiphoton microscope
A) Schematic of experimental design and resulting histology 24 days after the rabies injection. Top left: a population of birthdate-matched (E14) cells in the hippocampus was targeted to express TVA and G by injecting an undiluted virus carrying Cre in utero and a large volume of the rabies helper virus carrying TVA and G in the adult hippocampus. A rabies virus carrying GCaMP6f was used to label cells providing monosynaptic input to this cell population. These inputs were then visualised via a prism implanted between the MEC (brain region marked in grey) and the cerebellum. Bottom left: confocal maximum intensity projection of the dorsal hippocampus showing cells expressing helper proteins and/or GCaMP6f. Image insets are single z-plane confocal images showing colocalization of antibody staining for a tag introduced by the helper virus and GCaMP6f introduced by the rabies virus in one example cell (i.e. a starter cell) and only GCaMP6f expression in a neighbouring cell (i.e. an input cell) highlighted with an arrow and a yellow dotted circle. Right: confocal maximum intensity projection showing input cells expressing GCaMP6f in the MEC. The white dotted line illustrates the impression made by the prism on the MEC. DG: dentate gyrus, Sub: subiculum. B) In vivo imaging example. Top left: imaging is performed with head-fixed mice under a two-photon <t>benchtop</t> <t>microscope.</t> Right: maximum intensity projection (across a 2-minute recording) of five cells in the MEC that provide monosynaptic input to the ipsilateral hippocampus 19 days after the rabies injection. Bottom left: ΔF/F traces of two cells with different activity onsets highlighted with corresponding colours in the image to the right.
Custom Built Laserscanning Multiphoton Microscope, supplied by Laserscanning Europe GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/photon+microscope/two+photon+laserscanning+microscope/pm35025341-59-5-4
Average 90 stars, based on 1 article reviews
custom-built laserscanning multiphoton microscope - by Bioz Stars, 2026-08
90/100 stars
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mom-type two-photon microscope  (Sutter Instrument)
90
Sutter Instrument mom-type two-photon microscope
A) Schematic of experimental design and resulting histology 24 days after the rabies injection. Top left: a population of birthdate-matched (E14) cells in the hippocampus was targeted to express TVA and G by injecting an undiluted virus carrying Cre in utero and a large volume of the rabies helper virus carrying TVA and G in the adult hippocampus. A rabies virus carrying GCaMP6f was used to label cells providing monosynaptic input to this cell population. These inputs were then visualised via a prism implanted between the MEC (brain region marked in grey) and the cerebellum. Bottom left: confocal maximum intensity projection of the dorsal hippocampus showing cells expressing helper proteins and/or GCaMP6f. Image insets are single z-plane confocal images showing colocalization of antibody staining for a tag introduced by the helper virus and GCaMP6f introduced by the rabies virus in one example cell (i.e. a starter cell) and only GCaMP6f expression in a neighbouring cell (i.e. an input cell) highlighted with an arrow and a yellow dotted circle. Right: confocal maximum intensity projection showing input cells expressing GCaMP6f in the MEC. The white dotted line illustrates the impression made by the prism on the MEC. DG: dentate gyrus, Sub: subiculum. B) In vivo imaging example. Top left: imaging is performed with head-fixed mice under a two-photon <t>benchtop</t> <t>microscope.</t> Right: maximum intensity projection (across a 2-minute recording) of five cells in the MEC that provide monosynaptic input to the ipsilateral hippocampus 19 days after the rabies injection. Bottom left: ΔF/F traces of two cells with different activity onsets highlighted with corresponding colours in the image to the right.
Mom Type Two Photon Microscope, supplied by Sutter Instrument, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/photon+microscope/mom+type+two+photon+microscope/bio_rxiv__2021__05__31__446404-218-3-14
Average 90 stars, based on 1 article reviews
mom-type two-photon microscope - by Bioz Stars, 2026-08
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ratio-based microscopic fluorescent spectrophotometer  (Photon Technology International Inc)
90
Photon Technology International Inc ratio-based microscopic fluorescent spectrophotometer
A) Schematic of experimental design and resulting histology 24 days after the rabies injection. Top left: a population of birthdate-matched (E14) cells in the hippocampus was targeted to express TVA and G by injecting an undiluted virus carrying Cre in utero and a large volume of the rabies helper virus carrying TVA and G in the adult hippocampus. A rabies virus carrying GCaMP6f was used to label cells providing monosynaptic input to this cell population. These inputs were then visualised via a prism implanted between the MEC (brain region marked in grey) and the cerebellum. Bottom left: confocal maximum intensity projection of the dorsal hippocampus showing cells expressing helper proteins and/or GCaMP6f. Image insets are single z-plane confocal images showing colocalization of antibody staining for a tag introduced by the helper virus and GCaMP6f introduced by the rabies virus in one example cell (i.e. a starter cell) and only GCaMP6f expression in a neighbouring cell (i.e. an input cell) highlighted with an arrow and a yellow dotted circle. Right: confocal maximum intensity projection showing input cells expressing GCaMP6f in the MEC. The white dotted line illustrates the impression made by the prism on the MEC. DG: dentate gyrus, Sub: subiculum. B) In vivo imaging example. Top left: imaging is performed with head-fixed mice under a two-photon <t>benchtop</t> <t>microscope.</t> Right: maximum intensity projection (across a 2-minute recording) of five cells in the MEC that provide monosynaptic input to the ipsilateral hippocampus 19 days after the rabies injection. Bottom left: ΔF/F traces of two cells with different activity onsets highlighted with corresponding colours in the image to the right.
Ratio Based Microscopic Fluorescent Spectrophotometer, supplied by Photon Technology International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/photon+microscope/ratio+based+microscopic+fluorescent+spectrophotometer/pm11316737-104-3-5
Average 90 stars, based on 1 article reviews
ratio-based microscopic fluorescent spectrophotometer - by Bioz Stars, 2026-08
90/100 stars
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microscopic laser raman spectral device pdp-320  (Photon Design Corporation)
90
Photon Design Corporation microscopic laser raman spectral device pdp-320
A) Schematic of experimental design and resulting histology 24 days after the rabies injection. Top left: a population of birthdate-matched (E14) cells in the hippocampus was targeted to express TVA and G by injecting an undiluted virus carrying Cre in utero and a large volume of the rabies helper virus carrying TVA and G in the adult hippocampus. A rabies virus carrying GCaMP6f was used to label cells providing monosynaptic input to this cell population. These inputs were then visualised via a prism implanted between the MEC (brain region marked in grey) and the cerebellum. Bottom left: confocal maximum intensity projection of the dorsal hippocampus showing cells expressing helper proteins and/or GCaMP6f. Image insets are single z-plane confocal images showing colocalization of antibody staining for a tag introduced by the helper virus and GCaMP6f introduced by the rabies virus in one example cell (i.e. a starter cell) and only GCaMP6f expression in a neighbouring cell (i.e. an input cell) highlighted with an arrow and a yellow dotted circle. Right: confocal maximum intensity projection showing input cells expressing GCaMP6f in the MEC. The white dotted line illustrates the impression made by the prism on the MEC. DG: dentate gyrus, Sub: subiculum. B) In vivo imaging example. Top left: imaging is performed with head-fixed mice under a two-photon <t>benchtop</t> <t>microscope.</t> Right: maximum intensity projection (across a 2-minute recording) of five cells in the MEC that provide monosynaptic input to the ipsilateral hippocampus 19 days after the rabies injection. Bottom left: ΔF/F traces of two cells with different activity onsets highlighted with corresponding colours in the image to the right.
Microscopic Laser Raman Spectral Device Pdp 320, supplied by Photon Design Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/photon+microscope/raman+spectroscopic+microscope/us09627150-30-2-17
Average 90 stars, based on 1 article reviews
microscopic laser raman spectral device pdp-320 - by Bioz Stars, 2026-08
90/100 stars
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software for controlling the two-photon microscope and pre-processing of the calcium imaging data  (SourceForge net)
90
SourceForge net software for controlling the two-photon microscope and pre-processing of the calcium imaging data
A) Schematic of experimental design and resulting histology 24 days after the rabies injection. Top left: a population of birthdate-matched (E14) cells in the hippocampus was targeted to express TVA and G by injecting an undiluted virus carrying Cre in utero and a large volume of the rabies helper virus carrying TVA and G in the adult hippocampus. A rabies virus carrying GCaMP6f was used to label cells providing monosynaptic input to this cell population. These inputs were then visualised via a prism implanted between the MEC (brain region marked in grey) and the cerebellum. Bottom left: confocal maximum intensity projection of the dorsal hippocampus showing cells expressing helper proteins and/or GCaMP6f. Image insets are single z-plane confocal images showing colocalization of antibody staining for a tag introduced by the helper virus and GCaMP6f introduced by the rabies virus in one example cell (i.e. a starter cell) and only GCaMP6f expression in a neighbouring cell (i.e. an input cell) highlighted with an arrow and a yellow dotted circle. Right: confocal maximum intensity projection showing input cells expressing GCaMP6f in the MEC. The white dotted line illustrates the impression made by the prism on the MEC. DG: dentate gyrus, Sub: subiculum. B) In vivo imaging example. Top left: imaging is performed with head-fixed mice under a two-photon <t>benchtop</t> <t>microscope.</t> Right: maximum intensity projection (across a 2-minute recording) of five cells in the MEC that provide monosynaptic input to the ipsilateral hippocampus 19 days after the rabies injection. Bottom left: ΔF/F traces of two cells with different activity onsets highlighted with corresponding colours in the image to the right.
Software For Controlling The Two Photon Microscope And Pre Processing Of The Calcium Imaging Data, supplied by SourceForge net, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/photon+microscope/software+for+controlling+the+two+photon+microscope+and+pre+processing+of+the+calcium+imaging+data/pmc09803349-252-7-16
Average 90 stars, based on 1 article reviews
software for controlling the two-photon microscope and pre-processing of the calcium imaging data - by Bioz Stars, 2026-08
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Image Search Results


A) Schematic of experimental design and resulting histology 24 days after the rabies injection. Top left: a population of birthdate-matched (E14) cells in the hippocampus was targeted to express TVA and G by injecting an undiluted virus carrying Cre in utero and a large volume of the rabies helper virus carrying TVA and G in the adult hippocampus. A rabies virus carrying GCaMP6f was used to label cells providing monosynaptic input to this cell population. These inputs were then visualised via a prism implanted between the MEC (brain region marked in grey) and the cerebellum. Bottom left: confocal maximum intensity projection of the dorsal hippocampus showing cells expressing helper proteins and/or GCaMP6f. Image insets are single z-plane confocal images showing colocalization of antibody staining for a tag introduced by the helper virus and GCaMP6f introduced by the rabies virus in one example cell (i.e. a starter cell) and only GCaMP6f expression in a neighbouring cell (i.e. an input cell) highlighted with an arrow and a yellow dotted circle. Right: confocal maximum intensity projection showing input cells expressing GCaMP6f in the MEC. The white dotted line illustrates the impression made by the prism on the MEC. DG: dentate gyrus, Sub: subiculum. B) In vivo imaging example. Top left: imaging is performed with head-fixed mice under a two-photon benchtop microscope. Right: maximum intensity projection (across a 2-minute recording) of five cells in the MEC that provide monosynaptic input to the ipsilateral hippocampus 19 days after the rabies injection. Bottom left: ΔF/F traces of two cells with different activity onsets highlighted with corresponding colours in the image to the right.

Journal: bioRxiv

Article Title: All-viral tracing of monosynaptic inputs to single birthdate-defined neurons in the intact brain

doi: 10.1101/2021.10.18.464781

Figure Lengend Snippet: A) Schematic of experimental design and resulting histology 24 days after the rabies injection. Top left: a population of birthdate-matched (E14) cells in the hippocampus was targeted to express TVA and G by injecting an undiluted virus carrying Cre in utero and a large volume of the rabies helper virus carrying TVA and G in the adult hippocampus. A rabies virus carrying GCaMP6f was used to label cells providing monosynaptic input to this cell population. These inputs were then visualised via a prism implanted between the MEC (brain region marked in grey) and the cerebellum. Bottom left: confocal maximum intensity projection of the dorsal hippocampus showing cells expressing helper proteins and/or GCaMP6f. Image insets are single z-plane confocal images showing colocalization of antibody staining for a tag introduced by the helper virus and GCaMP6f introduced by the rabies virus in one example cell (i.e. a starter cell) and only GCaMP6f expression in a neighbouring cell (i.e. an input cell) highlighted with an arrow and a yellow dotted circle. Right: confocal maximum intensity projection showing input cells expressing GCaMP6f in the MEC. The white dotted line illustrates the impression made by the prism on the MEC. DG: dentate gyrus, Sub: subiculum. B) In vivo imaging example. Top left: imaging is performed with head-fixed mice under a two-photon benchtop microscope. Right: maximum intensity projection (across a 2-minute recording) of five cells in the MEC that provide monosynaptic input to the ipsilateral hippocampus 19 days after the rabies injection. Bottom left: ΔF/F traces of two cells with different activity onsets highlighted with corresponding colours in the image to the right.

Article Snippet: Mice were imaged with a custom two-photon benchtop microscope (Femtonics, Hungary) or two-photon miniscope ( Zong et al ., 2017, 2021 ; Obenhaus et al ., 2021 ).

Techniques: Injection, In Utero, Expressing, Staining, In Vivo Imaging, Imaging, Microscopy, Activity Assay